Plasmid

Part:BBa_K4941104:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-12)


pylxp-ylER-EcYida


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6898
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1788
    Illegal XhoI site found at 2186
    Illegal XhoI site found at 5315
    Illegal XhoI site found at 5348
    Illegal XhoI site found at 10441
    Illegal XhoI site found at 10470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6898
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6898
    Illegal NgoMIV site found at 5503
    Illegal NgoMIV site found at 11327
    Illegal AgeI site found at 2665
    Illegal AgeI site found at 4445
    Illegal AgeI site found at 6273
    Illegal AgeI site found at 9856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1634
    Illegal BsaI site found at 9035
    Illegal BsaI site found at 10612
    Illegal BsaI.rc site found at 1049
    Illegal BsaI.rc site found at 2988
    Illegal BsaI.rc site found at 4768
    Illegal BsaI.rc site found at 10527
    Illegal BsaI.rc site found at 10810
    Illegal SapI site found at 1936
    Illegal SapI.rc site found at 757
    Illegal SapI.rc site found at 10058


Design Notes

none



Source

yarrowia lipolytica

References